Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes
نویسندگان
چکیده
Rat antisera raised against anti-nuclease antibodies from mouse strains A/J and SJL detect strain-specific idiotypic determinants related to the antigen-combining site. These antisera have been used to investigate the genetic linkage and strain distribution of the anti-nuclease idiotypes. Despite the existence of an H-2-linked immune response gene controlling the humoral response to nuclease, expression of the A/J anti-nuclease idiotype has been shown to be independent of genes in the H-2 region: the A/J idiotype was present in immune sera from strains A/J (H-2a) and A.BY (H-2b) but absent in sera from strains B10 (H-2b) and B10.A (H-2a). An analysis of the segregation of the A/J idiotype in offspring of the backcross (A/J x B10.A) x B10.A demonstrated linkage to the Ig-1e heavy chain allotype markers. In a small sample of backcross animals a very high apparent recombination frequency was observed, but further backcross analyses and progeny testing of putative recombinant animals will be required to substantiate this observation. Analysis of the A/J and SJL anti-nuclease idiotype markers in the BALB/c, CB.20, and BAB.14 strains indicate that these idiotypic markers may permit mapping of distinct variable region genes.
منابع مشابه
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Staphylococcal nuclease is a well-characterized protein antigen that has been used in our laboratory for a variety of studies on the genetic control of immune responses (1). Rat antisera detecting binding site-specific idiotypic determinants of anti-nuclease antibodies have been used to study the genetics of variable region genes (2-4). Thus, genes determining the predominant idiotype produced ...
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ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 145 شماره
صفحات -
تاریخ انتشار 1977